br Data In addition to make the figures in the

Data In addition, to make the figures in the text become clearer, all of the figures were processed by Photoshop 8.1 software. ChemOffice 2008 was used for drawing the structures of KY02111 red 1 and acid green 50 (Fig. 1).
Experimental design, materials and methods
Acknowledgements The authors gratefully acknowledge financial support from Sichuan Provincial Science & Technology Fund for Applied Fundamental Research (2016JY0080), Open Fund (CSPC2013-6) of Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province (China West Normal University), and the Education Department of Sichuan Province (12ZA171).
Specifications Table
Value of the data
Data Data Capillary blood has been shown to be an adequate matrix for measuring 25OHD [1[. Automated immunoassays routinely used by hospital central laboratories suffer from a lack of specificity that mass spectrometry offers [2].
Experimental design, materials and methods 100μL of a blank, consisting of charcoal-stripped plasma (cat. #1131-00) purchased from Biocell (Rancho Dominguez, CA, USA), sample, calibrator or control were transferred to glass tubes, and spiked with 250μL of a mixture of deuterated internal standards 25OHD3 (26,26,26,27,27,27-d6, IS1), 25OHD2 (26,26,26,27,27,27-d6, IS2) from Chemaphor Inc., (Ottawa, ON, Can) and 3-epi-25OHD3 (6,19,19-d3, IS3) from Sigma-Aldrich Canada (Oakville, ON, Can) mixed gently for 10s, incubated for 1h at room temperature with periodic short mixing, before the addition of 1.0mL of 2-methoxy-2-methylpropane/hexane (50/50v/v). After a further 10-min incubation at room temperature, the mixture was centrifuged at 3000 RPM for 5min. 800μL of the supernatant was transferred to a 12/75-glass tube, evaporated to dryness at 37°C under a stream of N2. The crude extract was reconstituted in 100μL of the initial HPLC mobile phase (see below) and placed on the auto-sampler. The detailed chromatography and optimized+ion mode ESI-MS/MS conditions and ionic transition masses used are described in Table 1. HPLC/ESI MS-MS (high performance liquid chromatography/electrospray ionization tandem mass spectrometry) was performed on an Agilent 1200 HPLC system, consisting of degassers, binary and quaternary pumps, an auto-sampler equipped with a micro Rheodyne valve, and a temperature controlled column compartment with a column-switching valve coupled to an Agilent 6460 triple quadrupole mass spectrometer equipped with a JetStream™ interface (Agilent Technologies Canada Inc., Mississauga, ON, Canada). For the chromatography, 10μl of the extracts were injected on a jacket-heated (50°C) column [100×2.1mm Kinetex™ 2.6µm PentaFluoroPhenyl Core shell Silica 100Å (Phenomenex, Torrance, CA, USA)] preceded by a 2.1mm internal diameter (ID) SecurityGuard™ ULTRA cartridge (Phenomenex). The MassHunter workstation software, version B.04.00 (Agilent Technologies) was used for the management of the HPLC/ESI MS-MS system and data acquisition. The spectral analysis was done in the Multiple Reaction Mode (MRM). Fig. 1 illustrates representative selected ion LC-MS/MS chromatographic profiles of spiked charcoal-stripped plasma spiked with vitamin D standards and deuterated internal standards, and a patient serum extract analyzed in the conditions described above.
Acknowledgments
Data This data consist of the optimal derivatization condition of MI, including amount of derivatization reagent, derivative temperature and derivative time (Figs. 1 and 2). Furthermore, the detection volume of the plasma was minimized (Table 1).
Experimental design, materials and methods
Acknowledgments
Data Four figures and one table were provided to show the PDMS/spherical TiO2 nanocomposite characterization and investigation of data for applying as environmentally friendly marine antifouling paints. A schematic representation of fouling resistance mechanism of the UV-irradiated silicone nanocomposites is presented here.