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br Materials and methods br Results Teeth were collected fro
2018-11-08
Materials and methods Results Teeth were collected from 10 female and 6 male subjects. The subject ages ranged from 6 to 13years old, with an average age of 9. All subjects were typically developing children with no suspicion of undiagnosed neurogenetic disorders. The teeth included in the stu
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br Techniques to achieve appropriate expression of
2018-11-08
Techniques to achieve appropriate expression of designer nucleases in pluripotent stem cells The technique of nuclease transfer into the Autophagy Compound Library is not only critical in terms of efficiency but also with regard to safety issues, and has a profound impact on the targeting effici
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The karyotype of the BIOT LOAD
2018-11-08
The karyotype of the BIOT-4828-LOAD iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). Expression of pluripotency markers was examined by immunocytochemistry staining, using gingerol against human OCT3/4, E-CADHERI
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These results with fresh non fractionated not
2018-11-08
These results with fresh, non-fractionated, not culture-expanded, adipose tissue-derived SVF are comparable with other two previously reported clinical series, using cultured adipose-derived ADSCs with controlled cell doses. The adipose tissue contains large populations of mesenchymal procyanidin b2
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Previously we succeeded in visualizing the DNA methylation s
2018-11-08
Previously, we succeeded in visualizing the DNA methylation status by injecting a GFP-fused MBD of MBD1 protein (EGFP-MBD-NLS) mRNA into living mice zygotes. Using this method, we visualized the DNA methylation dynamics in preimplantation mouse embryos and found that DNA hypomethylation of major and
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br Results br Discussion The selfish
2018-11-08
Results Discussion The selfish spermatogonia selection hypothesis proposes that pathogenic ep4 receptor in human PAE disorders become enriched through clonal expansion of mutant SSCs that possess an acquired competitive advantage (Goriely and Wilkie, 2012). Here, we modeled stem cell fitness
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Single cell expression profiling has
2018-11-08
Single-cell expression profiling has allowed researchers to test the idea that gene expression variability reflects true biological variation in cellular mRNA levels. For example, in an analysis of individual pancreatic islet cells, the transcripts of insulin genes Ins1 and Ins2 were highly correlat
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br Materials and methods br Acknowledgments We
2018-11-08
Materials and methods Acknowledgments We would like to thank Dr. Saran Karumbayaram for providing guidance in the culture and expansion of the primate fibroblasts. We would also like to thank Steven Dowdy for depositing the T7-VEE-OKS-iG plasmid (Addgene). This project was funded by NIH grant
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Since the discovery of these cells Ingram et al
2018-11-08
Since the discovery of these tnf alpha inhibitors (Ingram et al., 2004), secondary colonies have been generated in several studies to assess the immaturity of ECFCs from blood or vascular wall and hierarchize them (Alphonse et al., 2015; Ingram et al., 2005; Patel et al., 2016). The aim of this wor
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Hematopoietic stem cells HSCs maintain the turnover of red
2018-11-08
Hematopoietic stem semagacestat (HSCs) maintain the turnover of red blood cells, platelets, and immune cells. They reside in the bone marrow, where several cell types contribute to their regulation (Lo Celso et al., 2011; Morrison and Scadden, 2014). How the concerted action of multiple niche compo
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br Introduction Embryonic stem cells ESCs
2018-11-07
Introduction Embryonic stem cells (ESCs) are karyotypically normal, self-renewing cell lines, derived from the inner cell mass (ICM) of the pre-implantation embryo (Evans and Kaufman, 1981; Martin, 1981). ESCs can be derived and expanded using a variety of conditions, including culture with the c
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purchase isradipine The naive to lineage primed transition w
2018-11-07
The naive to lineage primed transition was proposed as a gradual continuum of pluripotent states with distinct functional and transcriptional signatures and biases (Tesar, 2016). Several stem cell types with different degrees of pluripotency can be established under artificial culture conditions (Wu
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Blebbistatin We are interested in testing
2018-11-07
We are interested in testing the existence of multiple regime dynamics in industrial productivity growth across countries. Therefore, the whole point in this paper is to allow the convergence β to vary. We estimate the following equation: Note that we did not include country dummies. This way, our
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The second strand is the literature on capture Laffont
2018-11-07
The second strand is the literature on capture. Laffont and Tirole (1991) developed the three-layer model to study collusion between a privately-informed agent and intermediates employed by the principal to bridge the informational gap. They showed that the principal may benefit from this arrangemen
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The analysis of age specific recommended sleep durations
2018-11-07
The analysis of age-specific recommended sleep durations revealed similar results: the significantly highest proportion of patients with non-recommended low sleep duration (43%) was found among patients with SUD (AND 24%; SCZ 10%; c.f. Table 2). No significant gender differences were found with res
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