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In this issue of report
2018-11-09
In this issue of , report a study which used the publicly available data from the American Gut Project including questionnaires and fecal 16S rRNA sequence data. The report focuses on associations between self-reported allergies and the adult fecal microbiota composition. Self-reported allergy prev
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The following are the supplementary data
2018-11-08
The following are the supplementary data related to this article. Acknowledgments We thank Kati Hansen and Robert Kuhnert for excellent technical assistance and Rolf Kemler for helpful discussions and sharing reagents. We are grateful to Benoît Kanzler and Caroline Johner from the transgenic an
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Netrin G NTNG part of the netrin
2018-11-08
Netrin-G1 (NTNG1; +2.2), part of the netrin/DCC/neogenin pathway, not only is an attractive guidance molecule for enteric neurons (Jiang et al., 2003), but also is involved in the outgrowth of thalamocortical neurons (Lin et al., 2003). Mutations in netrin-G1 are uncommon causes of the Rett syndrome
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Yamazaki et al showed that PTCH receptor expression was obse
2018-11-08
Yamazaki et al. showed that PTCH receptor expression was observed in cultured human peripheral blood-derived endothelial progenitor cells (EPCs) but not in mature endothelial cells (Yamazaki et al., 2008), suggesting that SHh might have a certain effect on human EPCs and CD34+ cells. Indeed, recent
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br Materials and methods br Results Teeth were collected fro
2018-11-08
Materials and methods Results Teeth were collected from 10 female and 6 male subjects. The subject ages ranged from 6 to 13years old, with an average age of 9. All subjects were typically developing children with no suspicion of undiagnosed neurogenetic disorders. The teeth included in the stu
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br Techniques to achieve appropriate expression of
2018-11-08
Techniques to achieve appropriate expression of designer nucleases in pluripotent stem cells The technique of nuclease transfer into the Autophagy Compound Library is not only critical in terms of efficiency but also with regard to safety issues, and has a profound impact on the targeting effici
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The karyotype of the BIOT LOAD
2018-11-08
The karyotype of the BIOT-4828-LOAD iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). Expression of pluripotency markers was examined by immunocytochemistry staining, using gingerol against human OCT3/4, E-CADHERI
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These results with fresh non fractionated not
2018-11-08
These results with fresh, non-fractionated, not culture-expanded, adipose tissue-derived SVF are comparable with other two previously reported clinical series, using cultured adipose-derived ADSCs with controlled cell doses. The adipose tissue contains large populations of mesenchymal procyanidin b2
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Previously we succeeded in visualizing the DNA methylation s
2018-11-08
Previously, we succeeded in visualizing the DNA methylation status by injecting a GFP-fused MBD of MBD1 protein (EGFP-MBD-NLS) mRNA into living mice zygotes. Using this method, we visualized the DNA methylation dynamics in preimplantation mouse embryos and found that DNA hypomethylation of major and
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br Results br Discussion The selfish
2018-11-08
Results Discussion The selfish spermatogonia selection hypothesis proposes that pathogenic ep4 receptor in human PAE disorders become enriched through clonal expansion of mutant SSCs that possess an acquired competitive advantage (Goriely and Wilkie, 2012). Here, we modeled stem cell fitness
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Single cell expression profiling has
2018-11-08
Single-cell expression profiling has allowed researchers to test the idea that gene expression variability reflects true biological variation in cellular mRNA levels. For example, in an analysis of individual pancreatic islet cells, the transcripts of insulin genes Ins1 and Ins2 were highly correlat
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br Materials and methods br Acknowledgments We
2018-11-08
Materials and methods Acknowledgments We would like to thank Dr. Saran Karumbayaram for providing guidance in the culture and expansion of the primate fibroblasts. We would also like to thank Steven Dowdy for depositing the T7-VEE-OKS-iG plasmid (Addgene). This project was funded by NIH grant
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Since the discovery of these cells Ingram et al
2018-11-08
Since the discovery of these tnf alpha inhibitors (Ingram et al., 2004), secondary colonies have been generated in several studies to assess the immaturity of ECFCs from blood or vascular wall and hierarchize them (Alphonse et al., 2015; Ingram et al., 2005; Patel et al., 2016). The aim of this wor
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Hematopoietic stem cells HSCs maintain the turnover of red
2018-11-08
Hematopoietic stem semagacestat (HSCs) maintain the turnover of red blood cells, platelets, and immune cells. They reside in the bone marrow, where several cell types contribute to their regulation (Lo Celso et al., 2011; Morrison and Scadden, 2014). How the concerted action of multiple niche compo
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br Introduction Embryonic stem cells ESCs
2018-11-07
Introduction Embryonic stem cells (ESCs) are karyotypically normal, self-renewing cell lines, derived from the inner cell mass (ICM) of the pre-implantation embryo (Evans and Kaufman, 1981; Martin, 1981). ESCs can be derived and expanded using a variety of conditions, including culture with the c
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